Quick bioassay test from tillers for detecting ALS herbicide resistance of weedy rice and barnyardgrass

Resistance to acetolactate synthase (ALS) inhibitors have increased recently in South Brazil where the major weeds of flooded rice (barnyardgrass and weedy rice) evolved resistance imazapyr+imazapic. The aim this research was evaluate a growth medium for tissue regeneration tillers barnyardgrass, as well an agar-based bioassays test (also from tillers) detect susceptible resistant biotypes barnyardgrass imazapyr+imazapic vitro. Greenhouse experiments were conducted ALS-resistant (R) (S) biotypes, carried out adequate tiller determine concentration herbicide distinguish R S plants. culture that provided suitable MS 50% with addition benzylamino-purine. vitro containing allowed discriminate between required satisfactory control explants grown 0.9 μM 1.3 herbicide, respectively. bioassay using provides opportunity predict effectively rice.